serp1 primary antibody  (Proteintech)

Journal: Molecular Medicine Reports

Article Title: SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β-catenin/TCF/LEF signaling pathway

doi: 10.3892/mmr.2022.12709

Figure Lengend Snippet: Establishment of an inchoate acute hepatic injury model and detection of ERS-related proteins. (A) Pathological changes of tissues of mice in the model group detected by H&E staining. (B) Levels of alanine aminotransferase and aspartate aminotransferase in the serum. (C) ERS-related protein expression detected by western blotting. SERP1 expression in acute hepatic injury tissues detected by (D) immunohistochemistry and (E) western blotting. (F) SERP1 expression in acute hepatic injury cells detected by western blotting and reverse transcription-quantitative PCR. *P<0.05, **P<0.01 and ***P<0.001 vs. the control; n≥3. ERS, endoplasmic reticulum stress; SERP1, stress-associated endoplasmic reticulum protein 1; ALT, alanine aminotransferase; AST, aspartate aminotransferase; LPS, lipopolysaccharide; D-GalN, D-galactosamine; GRP78, glucose-regulated protein 78; GRP94, glucose-regulated protein 94.

Article Snippet: Subsequently, paraffin sections were washed with xylene, rehydrated with descending alcohol and antigen retrieval was performed at 95°C for 20 min. Then, 5% goat serum (Beijing Solarbio Technology Co. Ltd.) was used for blocking for 10 min at room temperature and sections were then incubated with the SERP1 primary antibody (1:200; cat. no. DF9873; Affinity Biosciences) at 4°C overnight.

Techniques: Staining, Expressing, Western Blot, Immunohistochemistry, Reverse Transcription, Real-time Polymerase Chain Reaction, Control

Journal: Molecular Medicine Reports

Article Title: SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β-catenin/TCF/LEF signaling pathway

doi: 10.3892/mmr.2022.12709

Figure Lengend Snippet: Effect of SERP1 overexpression on the expression of inflammatory factors in hepatocytes. Expression levels of SERP1 following transfection with SERP1 plasmid detected by (A) western blotting and (B) reverse transcription-quantitative PCR. **P<0.01, ***P<0.001 vs. OE-NC. (C) Expression levels of inflammatory factors (TNF-α, IL-18, IL-6 and IL-1β) detected by ELISA. (D) NLRP3 inflammasome (NLRP3, ASC and caspase-1) expression detected by western blotting. ***P<0.001 vs. the control; # P<0.05, ### P<0.001 vs. LPS + NC; Δ P<0.05, ΔΔ P<0.01 vs. LPS + SERP1; n≥3. SERP1, stress-associated endoplasmic reticulum protein 1; OE, overexpression; NC, negative control; NLRP3, NLR family pyrin domain containing 3; ASC, apoptosis-associated speck-like protein containing a CARD; LPS, lipopolysaccharide.

Article Snippet: Subsequently, paraffin sections were washed with xylene, rehydrated with descending alcohol and antigen retrieval was performed at 95°C for 20 min. Then, 5% goat serum (Beijing Solarbio Technology Co. Ltd.) was used for blocking for 10 min at room temperature and sections were then incubated with the SERP1 primary antibody (1:200; cat. no. DF9873; Affinity Biosciences) at 4°C overnight.

Techniques: Over Expression, Expressing, Transfection, Plasmid Preparation, Western Blot, Reverse Transcription, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control, Negative Control

Journal: Molecular Medicine Reports

Article Title: SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β-catenin/TCF/LEF signaling pathway

doi: 10.3892/mmr.2022.12709

Figure Lengend Snippet: Effect of SERP1 overexpression on apoptosis of hepatocytes. (A) A TUNEL assay was employed to detect cell apoptosis. (B) Apoptosis-related protein expression was detected by western blotting. ***P<0.001 vs. the control; # P<0.05, ## P<0.01, ### P<0.001 vs. LPS + NC; Δ P<0.05 vs. LPS + SERP1; n≥3. SERP1, stress-associated endoplasmic reticulum protein 1; LPS, lipopolysaccharide; NC, negative control.

Article Snippet: Subsequently, paraffin sections were washed with xylene, rehydrated with descending alcohol and antigen retrieval was performed at 95°C for 20 min. Then, 5% goat serum (Beijing Solarbio Technology Co. Ltd.) was used for blocking for 10 min at room temperature and sections were then incubated with the SERP1 primary antibody (1:200; cat. no. DF9873; Affinity Biosciences) at 4°C overnight.

Techniques: Over Expression, TUNEL Assay, Expressing, Western Blot, Control, Negative Control

Journal: Molecular Medicine Reports

Article Title: SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β-catenin/TCF/LEF signaling pathway

doi: 10.3892/mmr.2022.12709

Figure Lengend Snippet: Effect of SERP1 overexpression on endoplasmic reticulum stress-related protein expression and GSK3β/β-catenin/TCF/LEF signaling in hepatocytes. (A) Expression levels of GRP78, GRP94 and CHOP in hepatocytes detected by western blotting. (B) TOP Flash/FOP Flash fluorescent gene reporter assay to detect TCF/LEF activity. (C) GSK3β/β-catenin expression was detected by western blotting. ***P<0.001 vs. the control; # P<0.05, ## P<0.01, ### P<0.001 vs. LPS + NC; ΔΔΔ P<0.001 vs. LPS + SERP1; n≥3. SERP1, stress-associated endoplasmic reticulum protein 1; GRP78, glucose-regulated protein 78; GRP94, glucose-regulated protein 94; TCF, T-cell factor; LEF, lymphoid enhancing factor; LPS, lipopolysaccharide; NC, negative control.

Article Snippet: Subsequently, paraffin sections were washed with xylene, rehydrated with descending alcohol and antigen retrieval was performed at 95°C for 20 min. Then, 5% goat serum (Beijing Solarbio Technology Co. Ltd.) was used for blocking for 10 min at room temperature and sections were then incubated with the SERP1 primary antibody (1:200; cat. no. DF9873; Affinity Biosciences) at 4°C overnight.

Techniques: Over Expression, Expressing, Western Blot, Reporter Assay, Activity Assay, Control, Negative Control

Journal: Molecular Medicine Reports

Article Title: SERP1 reduces inchoate acute hepatic injury through regulation of endoplasmic reticulum stress via the GSK3β/β-catenin/TCF/LEF signaling pathway

doi: 10.3892/mmr.2022.12709

Figure Lengend Snippet: GSK3β/β-catenin signaling activation reverses the effect of SERP1 overexpression on ERS and cell apoptosis. (A) Overexpression was verified by reverse transcription-quantitative PCR and western blotting. ***P<0.001 vs. OE-NC; n≥3. (B) TCF/LEF activity was detected using a luciferase assay. (C) GSK3β/β-catenin expression was detected by western blotting. (D) Western blotting was applied to detect ERS-related protein expression. (E) A TUNEL assay was employed to detect cell apoptosis. (F) Apoptosis-related protein expression was detected by western blotting. *P<0.05, **P<0.01, ***P<0.001 vs. LPS; # P<0.05, ## P<0.01, ### P<0.001 vs. LPS + SERP1 + NC; n≥3. SERP1, stress-associated endoplasmic reticulum protein 1; ERS, endoplasmic reticulum stress; OE, overexpression; TCF, T-cell factor; LEF, lymphoid enhancing factor; LPS, lipopolysaccharide; NC, negative control.

Article Snippet: Subsequently, paraffin sections were washed with xylene, rehydrated with descending alcohol and antigen retrieval was performed at 95°C for 20 min. Then, 5% goat serum (Beijing Solarbio Technology Co. Ltd.) was used for blocking for 10 min at room temperature and sections were then incubated with the SERP1 primary antibody (1:200; cat. no. DF9873; Affinity Biosciences) at 4°C overnight.

Techniques: Activation Assay, Over Expression, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Activity Assay, Luciferase, Expressing, TUNEL Assay, Negative Control